Isolation and Molecular Characterization of Bacteria from Petroleum Soil

Chivukula L Gayitri; Puvvada K Anusha; Siddique A

Authors

Chivukula L Gayitri College of Agriculture, Family Sciences and Technology, Fort Valley State University, Georgia, USA.
Puvvada K Anusha Bioserve Biotechnologies Private Limited, Hyderabad, Telangana, India. https://orcid.org/0000-0002-4688-9904
Siddique A College of Agriculture, Family Sciences and Technology, Fort Valley State University, Georgia, USA.

Keywords:

Amplification, Centrifuge, Elution, Ethidium bromide, Primer, Safranine

Abstract

This study examined the types of bacteria present in petroleum soil. To identify the species we isolated DNA from bacteria samples. By serial dilution of soil sample we cultured bacteria. Bacterial isolation by using cultures. Bacterial DNA samples were subjected to PCR to get amplification. Extracted the DNA from agarose gel then sequenced the samples, which includes both forward and reverse sequencing. Sequencing results were put in bioinformatics software called BLAST gives the name of the microorganisms.

How to cite this article: Gayitri CL, Anusha PK, Siddique A. Isolation and Molecular Characterization of Bacteria from Petroleum Soil. J Adv Res Biology 2019; 1(2): 23-26.