Analgesic and Anti-Inflammatory Activities of The Ethanol Leaf Extract of Justicia Insularis (T. Anders) on Albino Rats
Keywords:
Justicia insularis, Analgesic anti-inflammation, Acetyl Salicyclic acid (ASA)Abstract
The ethanolic leaf extract of Justicia insularis (T. Anders) was evaluated for its analgesic and anti-inflammatory properties using acetic acid-induced writhing and thermal (hot plate) methods for analgesic activity and egg albumen-induced oedema as a model of acute inflammation respectively. 100mg/kg Bodyweight per day of Acytyl Salicyclic acid. (ASA) was used as a standard drug for analgesic. Phytochemical screening of the plant extract gave positive tests for saponins, alkaloids, tannins, flavonoids, anthraquinones and cardiac glycosides. The extract was dose-dependent and significantly at (P = 0.05). The median Lethal dose (LD50) was determined by intraperitoneal route which was calculated to be 2,449.49mg/kg using Lorke method. The experimental design used was a complete randomized type for all the studied parameters. The result further justified the use of J. insularis in herbal medicine for treatment of pains.
How to cite this article: Oyomah GO, Odoemena CS, Ogede RO et al. Analgesic and Anti-Inflammatory Activities of the Ethanol Leaf Extract of Justicia Insularis (T. Anders) on Albino Rats. J Adv Res Biology 2019; 1(2): 1-6.
References
Aguwa CN. Malaria, In: The Therapeutic Basis of Clinical Pharmacy in the Tropics 2nd ed. New York: Macmillan, 1996; 120-190.
Bohm BA, Kocipal-Abyazam R. Flavonoids and Condensed Tannins from leaves at Hawaliam Vaccinium Vaticulatum and V. Calycinium. Pacific Science 1994; 48: 458-463.
Bassey ME, Etuk EU, Bala D et al. Phytochemical and Nutritional Assessment of Justicia insularis. Journal of Environmental Design 2004; 2(1): 41-42.
Costa S, Jossary A, Bodo B. Phytochemcistry and Biological Properties of Kalanchoe Pinnata : Kalanchoe of Madagascar Systematic Ecophysiology and Phytochemistry. Paris: Kathala, 1995; 219–235.
Etuk EUI, Bassey ME, Ekpanyong DC et al. Quality Assessment of two Rural Infant Dieting Vegetables. The Industrial Journal of Nutritional Dietet 1997; 34: 5–7.
Gyang SS, Nyam DD, Sokomba EN. Hypoglycaemic Activity of Vernonia amygdalina (chloroform extract) in normaglycaemic and alloxan – induced hyperglycaemic rats. Journal of Pharmacy and Bioresources 2004; 1: 61–66.
Hughes JW, Smith T, Kosterllitz H. The Effects of Opoids Morphine on Humans Pain. 1975; 16: 134–142.
Hutchinson J, Dalziel JM. Flora of West Tropical Crown Agents for Overseas Government, London. 1958; 1(2): 110–120.
Koster R, Anderson M, De-Beer EJ. Acetic Acid for Analysis Screening Federation Proceeding, 1959; 18: 412.
Lewis T, Mansour A, Watson SJ et al. Opoids and Pain Regulation Stet. Pharmacentical Biology 1987; 22: 129–159.
Lork D. A New Approach to Practical Acute Toxicity Testing. Arch Toxicology 1983; 54: 275 -287.
Naggar EMB, Bartosikora L, Zemilicka M et al. Antidiabetic Effect of Eleome droserifolia Aerial Parts : Lipid Peroxidation – induced Oxidative Stress in Diabetic Rats. Acta Vet Brno 2005; 74: 347–352.
Odoemena CS, Essien JP. Antibacterial Activiity of the Root Extract of Telifairia Occidentalis. West African Journal of Biological and Applied Chemitry 1995; 40: 1-4.
Odoemena CS, Sampson AE, Bala D et al. Phytochemical Study and N u t r i t i v e potential of Afrofritomia sylvestis (Justicia isularis) Leaf. Nigeria Journal of Natural Products and Medicine 2002; 6: 52–54.
Sofowora EA. Medicinal Plants and Traditional Medicine in Africa. Spectrum Books Ltd. Ibadan, Nigeria. 1993; 289.
Stray F. The natural Guide to Medicinal Herbs and Plants. 2nd edn. London: Tiger Books International, 1998; 22–29.
Trease GE, Evans WC. A Textbook of Pharmacognosy 13th edn. Bailliere tindall Ltd, London. 1989; 40–58, 224–232.
Trease GE, Evans WC. Textbook of Pharmacognosy 14th edn Bailliere Tindal, London. 1999; 225-255.
Ugochukwu NH, Babvady NE, Cobourne M et al. The Effect of Gongronema latifolium Extract in Serum Lipid Profile and Oxidative stress in Hepatocytes of Diabetic Rats. Journal of Bioscience 2003; 28(1): 1–5.
Williams RF. Statistical Methods for the Analysis of Biochemical Data, Probability and Mathematical Statistics, Wiley: Chichester, 1987; 315-318.
Walker P, Whittlesea C. Clinical Pharmacy and Therapeutics. 4th edition, Eliservier Ltd. Philadelphia, 2007; 850-852.
Zaidi SH. Existing Indigenous Medicinal Plant Resources of Pakistan and their Prospectus for utilization. Pakistan Forest Journal 1998; 48(2): 5.
