Modified Extraction method and Characterization of sweet compounds from Stevia Rebaudiana

Authors

  • Jyoti Srivastava Advance Institute of Biotech & Paramedical Sciences, Naramau , Kanpur.
  • Mahroz . Advance Institute of Biotech & Paramedical Sciences, Naramau , Kanpur.
  • Padma S Vankar Facility for Ecological and Analytical Testing, Indian Institute of Technology, Kanpur 208016 India.

Keywords:

Stevia rebaudiana, Asteraceae, Lyophilization, HPLC-PDA, Stevioside, Rebaudioside a

Abstract

Stevia is a natural sweetener obtained from the leaves of Stevia rebaudiana (Bertoni), belonging to the
Asteraceae family. The objective of the present study was to develop a rapid and effective methodology
for the extraction of stevioside and rebaudioside A from Stevia leaves and to compare the yields with
different extraction techniques. Modified method of extraction was done with water finally the glycosides
were precipitated by methanol-water solvent (0.25:1.0 v/v). The solvent from the extract was removed
by lyophilization. Extracted product was analyzed by HPLC-PDA followed by mass-spectrometric (MS)
detections. The decolorized extract was separated on an NH2 column using PDA detection with excitation
at 210 nm. The mobile phase consisted of acetonitrile/water (80:20 v/v) with a flow rate of 1 ml/min.
Evaluative criteria for stevioside and rebaudioside yield in this new method was high. An improved
analytical method was developed which may be applied to quality control of stevioside content in dried
leaves of Stevia before processing.

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Published

2018-10-26

How to Cite

Srivastava, J., ., M., & Vankar, P. S. (2018). Modified Extraction method and Characterization of sweet compounds from Stevia Rebaudiana. Journal of Drug Discovery and Development ( ISSN:2581-6861), 1(1), 22-29. Retrieved from http://www.medicaljournalshouse.com/index.php/JDrug-Discovery-Development/article/view/50

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Vol. 1 No. 1 (2017): Journal of Drug Discovery and Development

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